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rplGB [2018-03-18 11:33:53]
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rplGB [2018-03-18 11:33:53]

highly expressed protein, similar to ribosomal protein L7AE family, associated with the ribosome during exponential growth, binds K-turns in RNA switches
locus
BSU01090
pI
9.51
mw
8.33 kDa
protein length
82 aa Sequence Blast
gene length
246 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
ybaB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

    • Gene

    Coordinates
    129,340 → 129,588

    The protein

    Catalyzed reaction/ biological activity

  • binds K-turns in [SW|RNA switch]es as they occur in the [protein|search|L-box], the [protein|search|S-box] and the [protein|search|T-box] [Pubmed|22355167]

    • Protein family

  • [SW|ribosomal protein] L7Ae family (according to Swiss-Prot)

    • Structure

  • [PDB|3V7E] ([protein|11D7ABA61FC4069B48614875AF2C6C694291B545|RplGB] bound to the [S-box|search|SAM-I riboswitch] aptamer) [Pubmed|22355167]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

    • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Mutant

  • BKE01090 (Δ[gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACATATCCTCCAAAG, downstream forward: _UP4_TAACGTACTTTTGTTTTTGC
  • BKK01090 (Δ[gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACATATCCTCCAAAG, downstream forward: _UP4_TAACGTACTTTTGTTTTTGC

    • References

  • 11948165,23249744,22355167